Dr.Nemani Harishankar


 Ph.D
Scientist ‘E’



Email:   hsnemani2000@yahoo.com
Contact:   +91-40-27197201
Brief Profile

He Joined ICMR in 1980 and spent initial years in the Food & Drug Toxicology Division of the Institute, wherein he worked on chemical, nutritional and toxicological evaluation of unconventional oils. He has obtained his Master’s Degree in the year 1988 in Applied Zoology from the Central University of Pondicherry. He moved to National Centre for Laboratory Animal Sciences (NCLAS) to pursue Ph.D in Zoology, which was awarded to him in 2002. His doctoral work was on the establishment and characterization of the obese mutant rats established at the centre. He is currently associated with the Animal Facility and is involved in all the centers activities like breeding and supply of animals as well as in training of personnel in handling lab animals both regular and adhoc. He has experience in working with different areas such as In vivo imaging of small animals, mutant breeding. He has been recognized as PhD research supervisor at Osmania University in Zoology. His areas of interest include animal physiology, animal behaviour, and also pre-clinical toxicology. He was instrumental in establishing the “State of the Art” animal physiology laboratory in the centre.

He serves an advisory committee member in health and nutrition for reputed Government bodies like the Department of Science and Technology (DST); Mid-day Meal Programme (MDM) and National Disaster Management Authority (NDMA).

 

Total Number of Publications: 48
  1. Sharvari Deshpande, Nemani Harishankar, Suresh Pothani, Nafisa Balasinor. Altered endocrine, cytokine signaling and oxidative stress: A plausible reason for differential changes in testicular cells in diet-induced and genetically-inherited – obesity in adult rats. Reproductive Biology. 2019.
  2. RamavatRavindar Naik, Harishankar Nemani, Suresh Pothani, Sailaja Pothana, Mota Satyavani, Syed SYH. Qadri, Myadara Srinivas, Brahmanaidu Parim. Obesity-alleviating capabilities of Acalypha indica, Pergulari ademia and Tinospora cardifolia leaves methanolic extracts in WNIN/GR-Ob rats. Journal of Nutrition & Intermediary Metabolism. 16 (2019) 100090. doi.org/10.1016/j.jnim.2019.02.001.
  3. V. V. Sathibabu Uddandrao,P. Rameshreddy,P. Brahmanaidu, Ponmurugan Ponnusamy,Santhanaraj Balakrishnan,Ravindar Naik Ramavat,K. Swapna,Suresh Pothani, Harishankar Nemani, Balaji Meriga,S. Vadivukkarasi, Nivedha P. R. and Saravanan Ganapathy. Anti-obesity efficacy of asiatic acid: down-regulation of adipogenic and inflammatory processes in high fat diet induced obese rats. doi.org/10.1080/13813455.2018.1555668.
  4. Sharvari S. Deshpande, Harishankar Nemani, Suresh Pothani , Kushaan Dumasia, Anita Kumar, Prathap Reddy Kallamadi, Nafisa H. Balasinor, 2018. Geneticallyinherited- and high fat diet induced- obesity deferentially alter spermatogenesis in adult male rats. Endocrinology. 2019 Jan 1;160(1):220-234. doi: 10.1210/en.2018-00569
  5. Swapna, V. V. Sathibabu Uddandrao, Brahmanaidu Parim, R. Ravindarnaik, P. Suresh, Ponmurugan Ponnusamy, Santhanaraj Balakrishnan, S. Vadivukkarasi, N. Harishankar, K. Prathap Reddy, P.R. Nivedha, Ganapathy Saravanan. Effects of Asiatic acid, an active constituent in Centella asiatica (L.): Restorative perspectives of Streptozotocin-Nicotinamide induced Changes on Lipid Profile and Lipid Metabolic Enzymes in Diabetic Rats. Comparative Clinical Pathology; 2019. (Accepted).
  6. Nemani shivaram, Kallamadi Prathap Reddy, Perumalla Kiran Kumar, Rayapoodi Naveen, Pothani Suresh, Nemani Harishankar. Unilateral Botulinum neurotoxin injection effects on growth, body compositgion and muscle conduction velocity in WNIN obese rats. Asian J Pharmacy and Pharmacology. 2018; 4(3):299-306.
  7. Karnam Shruthi, S.S Reddy, P. Y Reddy, P. Sivalingam, N. Harishankar and G.Bhanuprakash Reddy. Amelioration of neuronal cell death in a spontaneous obese rat model by dietary restriction through modulation of ubiquitin proteasome system. J.Nutr.Biochem. 2016 ;JNB-15-574R1.
  8. Shivaram Nemani, Uday K Putcha, Madhusudhanachary Perikety, Sailaja Potha, Giridharan Nappanveettil and Harishankar Nemani. Botulinum Neurotoxin effects on Masseter Muscle fibre in WNIN obese rats – Scanning Electron Microscope Analysis. 2015. Scanning. DOI: 10.1002/sca.21283.
  9. Hemalatha, R, Annapurna,V.V, Ramaraju A.V.S, Narendra Babu K, Thirupathaiah ,Y, Shujauddin Md. Harishankar. N, Balakrishna, N. Selective ceacal bacterial changes mediate the adverse effects associated with high palmolein or high starch diets: Prophylactic role of Flax oil. IJPPR. November 2015: Vol 7 Issue 11, pp. 89-95.
  10. Dinesh Thummuri, Manish K Jeengar, Shweta Shrivastava, Harishankar Nemani, Ravindar N Ramavat,  Pradip chaudhari, V.G.M.Naidu. Thymoquinone Inhibits RANKL Induced Osteoclastogenesis by Suppressing NF-KB and MAPK Signalling: A Study in Macrophage Cells and Mouse Model of Bone Loss. Pharmacol Res. 2015.99:63-73.
  11. Sd Muzammil Afzal, VGM Naidu, N. Harishankar and V. Kishan. Albumin Anchored Docetaxel Lipid Nanoemulsion For Improved Targeting E fficiency-Preparation, Characterization, Cytotoxic, Antitumor And In vivo Imaging Studies. Drug deliv 2015. 19: 1-9.
  12. Parim BrahmaNaidu, Nemani Harishankar, Balaji Meriga, Sailaja Pothana, Sajjalaguddam Ramgopalrao. Effects of Piper nigrum extract: Restorative perspectives of high fat diet induced changes on lipid profile, body composition and hormones in Sprague Dawley rats. Pharm Biol.  2015. 53 (9), 1318-13238.
  13. Prashanth Anamthathmakula Shanmugam M Jeyakumar Singotamu Lodhu, Harishankar Nemani,  Giridharan Nappan Veetil and Ayyalsomayajula Vajreswari. Mitochondriogenesis and apoptosis: possible cause of vitamin A-mediated adipose loss in WNIN/Ob-obese rats. Nutrition & Metabolism, Sep.2014. doi:10.1186/1743-7075-11-45.
  14. Yucang Li,Cule Wen, Dolly Mushahary, Ragamouni Sravanthi, Nemani Harishankar, Gopal pande, Peter Hodgson. Collagen type-I leads to in vivo matrix mineralization and secondry stabilization of Mg-Zr-Ca alloy implants. Colloids Surf B Biointerfaces. 2014 Oct 1;122:719-28. doi: 10.1016/j.colsurfb.2014.08.005. Epub 2014 Aug 19.
  15. Parim BrahmaNaidu, Harishankar Nemani, Balaji Meriga, Santosh kumar Mehar, Sailaja Pothana, Sajjalaguddam Ramgopalrao. Mitigating efficacy of piperine in the physiological derangments in high fat diet induced obesity in sprague dawley rats. Chemico Biological Interaction, 2014.  Jul 31;221C:42-51. doi: 10.1016/j.cbi.2014.07.008. (Epub ahead of print).
  16. D Mushahary, C Wen, JM Kumar, N Harishankar, G Pande, P Hodgson, Y Li. “Effect of Collagen type-I on the rate of osseointegration of Ca-containing biodegradable Mg-Zr based alloys” European Cells and Materials Vol. 26. Suppl. 6, 2013 (page 22) ISSN 1473-2262 http://www.ecmjournal.org.  
  17. Amit K. Jaiswal, Rohit V. Dhumal, Sandipto Ghosh, Pradip Chaudhari, Harishankar Nemani, Vivek P. Soni, Geeta R. Vanage and Jayesh R. Bellare. Bone healing evaluation of nanofibrous composite scaffolds in rat calvarial defects: A comparative study. Journal of Biomedical  Nanotechnology.2013. 9(12): 2073-2085 (13).
  18. Dolly Mushahary, Ragamouni Sravanthi, Jerald Mahesh, Nemani Harishankar, Gopal Pande. Zr, Ca and Sr Contents in Mg Based Biodegradable Alloys Modulate the Efficiency of Implant Induced Osseointegration. Int.J.Nanomedicine.2013. 8: 2887–2902.
  19. Sravanthi Ragamouni, Jerald Mahesh Kumar, Dolly Mushahary, Harishankar Nemani and Gopal Pande. Histological analysis of cells and matrix mineralizationof new bone tissue induced in rabbit femur bones by Mg-Zr based biodegradable implants. Acta Histochemica, 2013; Sep;115 (7):748-56.
  20. Anand Kumar Kalle, Lalitha Anumula, Pavithra D, Padmavathi I.J.N, Manisha Ganeshan Rajender Rao K, Venu Lagishetty, Balakrishna Nagalla, Harishanker Nemani, Umakar Reddy S, Giriraj Ratan Chandak, Shantanu Sengupta and Raghunath, M. Maternal dietary folate and / or vitamin b12 restrictions alter body composition (adiposity) and lipid metabolism in wistar rat offspring. J.Nutr.Biochem 2013; 24: 25-31.
  21. Nemani Harishankar and Nappanveettil Giridharan. Restoration of libido in WNIN/GR-Ob rats by administration of high dose of Testosterone propionate. Adv.Stud.Biol. 2012. 12:557-571.
  22. Siva Sanakara Vara Prasad Sakamuri, Mahesh Sukapaka, Vijay Kumar Prathipati, Harishankar Nemani, Uday Kumar Putcha, Shailaja Pothana, Swarupa Rani Koppala, Lakshmi Raj Kumar Ponday, Vani Acharya, Giridharan Nappanveettil, Vajreswari ayyalasomayajula. Carbenoxolone treatment ameliorated metabolic syndrome in WNIN/Ob obese rats, but induced severefat loss and glucose intolerance in lean rats. PLOS ONE; Dec. 2012; Vol 7; Issue 12; e50216.
  23. Yucang Li,Cule Wen, Dolly Mushahary, Ragamouni Sravanthi, Nemani Harishankar, Gopal pande, Peter Hodgson. Mg-Zr-Sr alloys as biodegradable implant materials. Acta Biomaterialia 8 2012. 3177–3188.
  24. Satishkumar,M. Y. Ali, P. Sailaja, S. Mahesh, M.V.Surekha, N.V.Giridharan and N.Harishankar. Therapeutic properties of Mucuna pruriens Linn – an Unani drug, in a prediabetic obese rat model. Int.J.Body comp res. 2012.10; 1:1-8.
  25. Nemani Harishankar, Eunni Seshadri, Subramanyam Kalyanasundaram and Nappanveettil Giridharan. Impaired glucose tolerance (IGT) to frank diabetes, by dietary manipulations in 4.552WNIN/GR-Ob rats. J. Diab. Mell. 2012. Vol.2. No.1. 52-58.
  26. Nemani Harishankar, Punjal Ravinder, K.Madhavan Nair and Nappanveettil Giridharan. Infertility in WNIN obese mutant rats – causes ?. ISRN Endocrinology, 2011c. Article ID 863403, 7 pages. doi:10.5402/2011/863403.
  27. N. Harishankar. A. Vajreswari,. N. V. Giridharan. WNIN/GR-Ob – An insulin-resistant obese rat model from inbred WNIN strain. Indian J Med Res. 2011; 134:320-329.
  28. Nemani Harishankar, Putcha Uday Kumar, Boindala Sesikeran and Nappanveettil Giridharan. Obesity associated pathophysiological & histological changes in WNIN obese mutant rats. Indian J Med Res. 2011; 134:330-340.
  29. Manisha Ganeshan, Sainath, P.B, Padmavathi, I.J.N, Venu Lagishetty, Kishore,Y.D, Kalle anand, Harishankar Nemani, Srinivasa Rao, J  and Raghunath Manchala. Maternal manganese restriction increases susceptibility to high fat diet induced dyslipidemia and altered adipose function in WNIN male rat offspring. Exp.Diab.Res 2011.
  30. I.J.N.Padmavathi, K.Rajender Rao, L.Venu,Ch.Narasimha Rao, N. Harishankar, Ayesha Ismail  and Manchala Raghunath.“Chroinic Dietary restriction modulates visceral adiposity probable underlying mechanism” Diabetes. 2010. 59:98-104.
  31. Padmavathi J.N Inagadapa, DurgaKishore Yedla, Venu Lagishetty, Manisha Ganeshan, Harishankar Nemani, Giridharan NV, and Raghunath Manchala. “Prenatal and Perinatal Zinc Restriction: Effects on body composition, glucose tolerance and insulin response in rat offspring” Exp.Physiol, 94:6, 761-769. 2009.
Professional Abstracts
  1. H. Nemani, U. Putchha, S. Pothan1, G. Nappanveettil. OXIDATIVE DAMAGE, AGEING AND DEGENERTIVE DISEASES IN WNIN OBESE RATS. Ann Nutr Metab. 2013;63(suppl 1):1–1960 1165 (Abstract).
  2. S. Manikonda, U K. Putchha, S. Pothana, S. Pothani,M. Murthy, S. Kovela, H. Nemani. ANTI-HYPERGLYCEMIC AND ANTI-OBESITY EFFECT OF ICHNOCARPUS FRUTESCENS, LINN.ON STREPTOZOTOCIN INDUCED DIABETIC RATS. Ann Nutr Metab 2013;63(suppl 1):1–1960. 1187 (Abstract).
  3. R N. Ramavat, H. Nemani, S. Pothani, N. Turlapati, B, R. Geereddy EFFECT OF ANDROGRAPHOLIDE ON STREPTOZOTOCIN-INDUCED DIABETIC CATARACT INSPRAGUE-DAWLEY RATS. Ann Nutr Metab 2013;63(suppl 1):1–1960, 1198 (Abstract).
  4. S.S.S.V.Prasad, N.Harishankar, S.Mahesh, P.Sailaja, N.V.Giridharan, A.Vajreswari et al., Presented a poster entitled “11Beta-Hydroxysteroid Dehydrogenase Type I inhibition amelirats metabolic syndrome in WNIN/Ob obese rats but induced glucose intolerance in leant rats” Steroid Research. P.1.33; 2011
  5. S.S.S.V.Prasad, N.Harishankar, N.V.Giridharan, A.Vajreswari. “11Beta-Hydroxysteroid Dehydrogenase Type I activity in liver and adipose tissue of WNIN/GR-Ob obese rats, A new genetic obese rat model with impaired glucose tolerance” Steroid Research. P.2.34 2011.
  6. Satish Kumar, A.Saleem, Mir Yousuf Ali, P.Sailaja, S.Mahesh, N.Harishankar, B.Dinesh Kumar and N.V.Giridharan. Hypoglycemic and hypolipidemic effect of M.pruriens on WNIN/GR-Ob rats. Ind.J.Pharmacol. 42 (Suppl.2). Abstracts. (PP 35), S233, 2010.
  7. Giridharan NV, Sailaja P and Harishankar N. A new obese rat model to study obesity and cardiovascular risks. CMJ@journal, 2010. (Abstract) 96 p.
  8. Harishankar N; Balakrishna N; Radhakrishna KV; Udaykumar P: Giridharan NV : Determination of body composition in Syrian hamsters by non-invasive methods. Ann Nutr Metab. 55 (Suppl 1). Abstracts. (P97- 02), p.470, 2009.
Popular Article

A popular article entitled “Determination of small animal body composition by TOBEC–A non-invasive method” contributed for NLCAS newsletter. Vol.33. October. 2006.

Awards and Honours
  1. Received Prof. Manjeeth Singh award for our work entitled “Carnosic acid Inhibits Osteoclastogenesis by Up Regulating the Nrf2 and Suppressing the NF-KB, MAPK Signaling” in Annual Conference of Indian Pharmacological Society, held at Guwahati from 28 th to 29 th December 2014.
  2. Received Prof. P.P.Suryakumari gold medal award for the best paper published on diabetes in 46 th Annual Conference of Indian Pharmacological Society, held at Bangalore from 16 th to 18 th , December 2013.
  3. Received UK LTD bursary award to attend 7 th Small Animal Imaging workshop held between 23 rd to 27 th of January 2012 at Tubingen University, Tubingen, Germany.
  4. Received Techniplast, Best poster award (sponsored trip to FELASA2010, at Helsinki, Finland) for our work entitled “WNIN/Ob, a Novel rat model to study oseoarthritis and osteoporosis” in the LASA conference held from 1 st - 3 rd October 2009, NIRRH, at Mumbai.
  5. Received Dr.K.R.Bhardwaj, Best poster award for our work entitled “WNIN/Ob, a Novel rat model to study oseoarthritis and osteoporosis” in the LASA conference held between 1 st -3 rd October 2009, NIRRH, at Mumbai.
  6. Received ICN 2009 (International Congress of Nutrition) travel award for the paper entitled “Determination body composition in Syrian hamsters by non-invasive methods” presented during 19 th International Congress of Nutrition, held between 4 th –10 th October 2009, BITEC, Bangkok, Thailand.
  7. Received CSIR best poster award In Experimental nutrition for the paper “Determination of body composition and activity in three commonly used rat strains” presented during the XXXVI Annual meetings of the Nutrition Society of India held between 5-6 th , Nov.2004 at CFTRI, MYSORE.
  8. Received young scientist award (Senior session) in Experimental nutrition for the paper presented during the XXXI Annual meetings of the Nutrition Society of India held on 26-28,Nov.1998 at National Institute of Nutrition, Hyderabad.